DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae)
نویسندگان
چکیده
منابع مشابه
Efficient DNA barcode regions for classifying Piper species (Piperaceae)
Piper species are used for spices, in traditional and processed forms of medicines, in cosmetic compounds, in cultural activities and insecticides. Here barcode analysis was performed for identification of plant parts, young plants and modified forms of plants. Thirty-six Piper species were collected and the three barcode regions, matK, rbcL and psbA-trnH spacer, were amplified, sequenced and a...
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Piper tuberculatum (Piperaceae) is a species that accumulates especially amides as secondary metabolites and several biological activities was previously reported. In this article, we report a proteomic study of P. tuberculatum. Bidimensional electrophoresis (2D SDS-PAGE) and mass spectrometry (ESI-Q-TOF) were used in this study. Over a hundred spots and various peptides were identified in this...
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Arbitrarily-primed DNA markers can be very useful for genetic fingerprinting and for facilitating positional cloning of genes. This class of technologies is particularly important for less studied species, for which genome sequence information is generally not known. The technologies include Randomly Amplified Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Amplified Fragmen...
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DNA-based techniques have great potential for detecting genetic variability between genotypes. However, each approach generates different molecular markers, so the kind and amount of polymorphism detected and the cost and time required vary among them. In general, a good method for DNA fingerprinting to detect genetic polymorphism must generate a complex pattern of bands per experiment, the res...
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ژورنال
عنوان ژورنال: Acta Botanica Brasilica
سال: 2018
ISSN: 1677-941X,0102-3306
DOI: 10.1590/0102-33062017abb0268